Molecular cloning of cDNA for Avena phytochrome
نویسندگان
چکیده
منابع مشابه
Molecular cloning of mouse placental lactogen cDNA.
We have isolated a cDNA clone for the 23-kDa mouse placental lactogen II (mPL-II) from a phage lambda gt11 expression library containing cDNA synthesized from BALB/c placental RNA. Translation in vitro of placental mRNA selected by hybridization to the mPL-II cDNA clones yields a 26-kDa polypeptide that is the size of the expected precursor protein and that is immunoprecipitated with anti-mPL-I...
متن کاملMolecular cloning of a canine metallothionein cDNA.
A canine metallothionein cDNA obtained from the liver of a cadmium-treated beagle was cloned and sequenced. Asn at position 4 conserved among all mammalian metallothionein-1 and metallothionein-2 is replaced by Asp in the canine metallothionein cDNA clone. Because the acidic amino acid doesn't exist at either position 10 or 11 in the deduced amino acid sequence, it is supposed that this cDNA is...
متن کاملMolecular cloning and characterization of cDNA for human myeloperoxidase.
Partial amino acid sequence of human myeloperoxidase was determined, and a 41-base oligonucleotide containing deoxyinosines at four positions was chemically synthesized. By using the oligonucleotide as a probe, cDNA clones for human myeloperoxidase were isolated from a cDNA library constructed with mRNA from human promyelocytic leukemia HL-60 cells. One of the clones containing a 2.6-kilobase i...
متن کاملMolecular Cloning of cDNA for Rat Liver Gap Junction Protein
An affinity-purified antibody directed against the 27-kD protein associated with isolated rat liver gap junctions was produced. Light and electron microscopic immunocytochemistry showed that this antigen was localized specifically to the cytoplasmic surfaces of gap junctions. The antibody was used to select cDNA from a rat liver library in the expression vector lambda gtll. The largest cDNA sel...
متن کاملMolecular cloning of cDNA for the human interleukin-2 receptor.
Interleukin-2 (IL-2 or T cell growth factor) is a 14,500 daltons glycoprotein critically involved in the development of a normal human immune response [1, 2J. Recently, cDNA for this lymphokine has been isolated and expressed in both prokaryotic and eukaryotic cells [3-5J. Further, the human IL-2 gene has been cloned, sequenced [6], and localized to chromosome 4. As with other polypeptide hormo...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1984
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.81.8.2332